Product Description

BCKDK (E41) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1869-50, BS1869-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14874

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP 1:50 - 1:100

Background: BCKDK (branched chain ketoacid dehydrogenase kinase), also known as BCKDHKIN, is a 412 amino acid mitochondrial matrix protein that exists as a monomer and contains one histidine kinase domain. Expressed ubiquitously, BCKDK catalyzes the ATP-dependent phosphorylation and subsequent inactivation of the branched-chain α-ketoacid dehydrogenase (BCKD) complex, a regulatory enzyme complex that plays a crucial role in the catabolic pathways of valine, leucine and isoleucine. Specifically, the BCKD complex functions as the second enzyme in branched-chain amino acid (BCAA) catabolism, effectively catalyzing the irreversible oxidative decarboxylation of BCAAs. Due to the ability of BCKDK to regulate the activity of the BCKD complex, BCKDK plays an essential role in the catabolic pathways of branched-chain amino acid metabolism.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BCKDK (E41) polyclonal antibody detects endogenous levels of BCKDK protein.

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BCKDK (E41) polyclonal antibody at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: AML-12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of BCKDK (E41) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50., Immunoprecipitation of HEK293T cell lysate using BCKDK (E41) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using BCKDK (E41) #BS1869. “↑”（supernatant); “↓(deposition）

Alternative Name: BCKD kinase; BCKD-kinase; BCKDHKIN; Bckdk; Branched chain alpha keto acid dehydrogenase kinase; Branched chain ketoacid dehydrogenase kinase; Branched-chain alpha-ketoacid dehydrogenase kinase; mitochondrial;

Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human BCKDK.

Conjugate: Unconjugated

Modification: Unmodified