Product Description

BCKDK (E41) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1869-50, BS1869-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: BCKDK (branched chain ketoacid dehydrogenase kinase), also known as BCKDHKIN, is a 412 amino acid mitochondrial matrix protein that exists as a monomer and contains one histidine kinase domain. Expressed ubiquitously, BCKDK catalyzes the ATP-dependent phosphorylation and subsequent inactivation of the branched-chain α-ketoacid dehydrogenase (BCKD) complex, a regμlatory enzyme complex that plays a crucial role in the catabolic pathways of valine, leucine and isoleucine. Specifically, the BCKD complex functions as the second enzyme in branched-chain amino acid (BCAA) catabolism, effectively catalyzing the irreversible oxidative decarboxylation of BCAAs. Due to the ability of BCKDK to regμlate the activity of the BCKD complex, BCKDK plays an essential role in the catabolic pathways of branched-chain amino acid metabolism.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: BCKD kinase, BCKD-kinase, BCKDHKIN, Bckdk, Branched chain alpha keto acid dehydrogenase kinase, Branched chain ketoacid dehydrogenase kinase, Branched-chain alpha-ketoacid dehydrogenase kinase, mitochondrial,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human BCKDK.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: BCKDK (E41) polyclonal antibody detects endogenous levels of BCKDK protein.

Molecular Weight: ~ 46 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14874

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only