Product Description

BID Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67031-50, MB67031-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax . Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane . Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resμlting in caspase-9 activation . Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF/ICC

Alternate Names: BH3-interacting domain death agonist, p22 BID, BID

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human BID. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of BID protein.

Molecular Weight: ~ 22 kDa

Pathway: Regμlation of Apoptosis, Death Receptor Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P55957

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only