Product Description

Bim (phospho-S69/S65) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64039-50, BS64039-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regμlating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resμlting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic peptide corresponding to residues in Human Bim around the phosphorylation site of Ser69.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Bim (S69/S65) polyclonal antibody detects endogenous levels of human Bim protein only when phosphorylated at Ser69. This antibody also recognizes mouse or rat Bim Protein when phosphorylated at Ser65.

Molecular Weight: ~ 25 kDa

Pathway: Regμlation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

SwissProt: O43521

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only