Bim Polyclonal Antibody-BS6459

Bim Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6459-50, BS6459-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43521

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 18KDa/23KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Bim antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunofluorescence analysis of NIH/3T3 cells using Bim Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using Bim Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using Bim Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: BAM; BIM; BOD; Bim; BCL2L11

Immunogen: A synthetic peptide of human Bim(NP_619527.1).

Conjugate: Unconjugated

Modification: Unmodified