BORA Polyclonal Antibody - E-AB-66144

BORA Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-66144-60, E-AB-66144-120, E-AB-66144-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: BORA

Target Synonym: BORA; C13orf34

Research Areas: Cancer, Cell Biology, Signal transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q6PGQ7

Background: Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm. Plk (polo-like kinase) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation on its N-terminus by ARK-1. It is through this mechanism that Bora and ARK-1 control cellular mitotic entry. Downregulation of the gene encoding Bora results in multipolar spindles in mitosis, a phenomenon that is also observed when ARK-1 function is blocked.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human BORA (NP_079084.3).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 53kDa/61kDa

Observed MW: 90kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only