BRAF Monoclonal Antibody - BT-MCA2288

BRAF Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA2288-50, BT-MCA2288-100

Citations, Manuals and MSDS Available upon request.

Background: BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

Research Areas: Cell Biology

Target Protein: BRAF

Applications: WB, IHC, ICC

Reactivity: Human, Mouse

Clonality: Monoclonal

Clone ID: 1H12

Host: Mouse

Isotype: Mouse IgG1

Full Product Name: BRAF

Immunogen: Purified recombinant fragment of human BRAF expressed in E. Coli.

Storage: 4°C|-20°C for long term storage

Formulation: Ascitic fluid containing 0.03% sodium azide.

Molecular Weight (Da): 87kDa

UniProt Accession: P15056

Synonyms: BRAF1, RAFB1, B-RAF1, FLJ95109

GeneID: 673

Research Use Only