Product Description

BUB1B (T368) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2244-50, BS2244-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60566

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB: 1:500~1:1000
IP: 1:50~1:200

Background: Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BUB1B (T368) polyclonal antibody detects endogenous levels of BUB1B protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BUB1B (T368) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate, Immunoprecipitation of HEK293T cell lysate using BUB1B (T368) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using BUB1B (T368) #BS2244. “↑”（supernatant）;“↓(deposition）

Alternative Name: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1

Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human BUB1B.

Conjugate: Unconjugated

Modification: Unmodified