Product Description

C/EBP β (phospho Thr235) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1275-50, ES1275-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CEBPB, LAP, TCF5, PP9092, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Nuclear factor NF-IL6, Transcription factor 5, TCF-5

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188. AA range:201-250

Isotype: IgG

Target: C/EBP β

Specificity: Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed at low levels in the lung, kidney and spleen,

Observed Band (KD): 36kD

Fields: IL-17 signaling pathway, TNF signaling pathway, Tuberculosis, Transcriptional misregulation in cancer

Function: Function: Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'
PTM: Sumoylated by polymeric chains of SUMO2 or SUMO3
Similarity: Belongs to the bZIP family
Similarity: Belongs to the bZIP family. C/EBP subfamily
Similarity: Contains 1 bZIP domain
Subunit: Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha, delta and gamma. Interacts with TRIM28 and PTGES2
Tissue Specificity: Expressed at low levels in the lung, kidney and spleen

Subcellular Location: Nucleus Cytoplasm Translocates to the nucleus when phosphorylated at Ser-288 In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: CEBPB

Protein Name: CCAAT/enhancer-binding protein beta

Mouse Gene ID: 12608

Mouse SwissProt Number: P28033

Rat Gene ID: 24253

Rat SwissProt Number: P21272

Research Use Only