Calnexin (phospho-S583) Polyclonal Antibody-BS4262

Calnexin (phospho-S583) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4262-50, BS4262-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27824

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:10000~1:50000
IHC: 1:50~1:1000
IP: 1:50~1:200

Background: Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Calnexin (S583) polyclonal antibody detects endogenous levels of Calnexin protein only when phosphorylated at Ser583

Bioworld Molecular Weight: ~ 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:10000 to 1:50000 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (40ug)
Lane 3: A375 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Calnexin (S583) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 whole cell lysate using Calnexin (phospho-S583) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 20% input. The western blot was probed using Calnexin (phospho-S583) pAb #BS4262. “↑”（supernatant); “↓”（deposition）

Alternative Name: Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX

Immunogen: Synthetic phosphopeptide derived from human Calnexin around the phosphorylation site of Serine 583.

Conjugate: Unconjugated

Modification: Phosphorylation