Product Description

CD110 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4199-50, ES4199-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MPL, TPOR, Thrombopoietin receptor, TPO-R, Myeloproliferative leukemia protein, Proto-oncogene c-Mpl, CD110

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from Thrombopoietin receptor at AA range: 321-370

Isotype: IgG

Target: CD110

Specificity: CD110 Polyclonal Antibody detects endogenous levels of CD110 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed at a low level in a large number of cells of hematopoietic origin, Isoform 1 and isoform 2 are always found to be coexpressed,

Observed Band (KD): 69,40kD

Fields: Cytokine-cytokine receptor interaction, JAK-STAT signaling pathway

Function: Caution: It is uncertain whether Met-1 or Met-8 is the initiator
Disease: Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM: 604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies
Domain: The box 1 motif is required for JAK interaction and/or activation
Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding
Function: Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses
Similarity: Belongs to the type I cytokine receptor family. Type 1 subfamily
Similarity: Contains 2 fibronectin type-III domains
Subunit: Interacts with ATXN2L
Tissue Specificity: Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and

Subcellular Location: Cell membrane, Single-pass type I membrane protein Golgi apparatus Cell surface

Storage and Stability: -20°C/1 year

Gene Name: MPL

Protein Name: Thrombopoietin receptor

Mouse Gene ID: 17480

Mouse SwissProt Number: Q08351

Research Use Only