Product Description

CD117 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66859-50, MB66859-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors . It plays a critical role in activation and growth in a number of cell types, including hematopoietic stem cells, mast cells, melanocytes, and germ cells . Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit resμlts in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components, including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL . Molecμlar lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders , and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors . Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF/ICC

Alternate Names: SCFR, Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human CD117. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of CD117 protein.

Molecular Weight: ~ 110 kDa

Pathway: Signaling Pathways Activating p38 MAP Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P10721

Product: Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only