Product Description

CD129 (Phospho-S519) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67585-50, BS67585-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q01113

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Plays an important role in the immune response against parasites by acting as a receptor of IL9.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD129 with a phosphorylation site at S519 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD129 (pS519) expression in HuT78 (A) whole cell lysates., Immunohistochemical analysis of CD129 (pS519) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of CD129 (pS519) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: Interleukin-9 receptor; IL-9 receptor; IL-9R; CD129

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD129 with a phosphorylation site at S519. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Phosphorylation