Product Description

CD148 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4130-50, ES4130-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PTPRJ, DEP1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J, CD148

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human PTPRJ. AA range:861-910

Isotype: IgG

Target: CD148

Specificity: CD148 Polyclonal Antibody detects endogenous levels of CD148 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in the promyelocytic cell line HL-60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the IL3 and erythropoietin-dependent leukemic cell line F-36E, Expressed predominantly in epithelial cells and lymphocytes, Enhanced expression at high cell density,

Observed Band (KD): 150kD

Fields: Adherens junction, Chemical carcinogenesis - reactive oxygen species

Function: Catalytic Activity: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Disease: Defects in PTPRJ are found in cancers of colon, lung, and breast
Function: May contribute to the mechanism of contact inhibition of cell growth
PTM: N- and O-glycosylated
Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily
Similarity: Contains 1 tyrosine-protein phosphatase domain
Similarity: Contains 9 fibronectin type-III domains

Subcellular Location: Cell membrane, Single-pass type I membrane protein Cell projection, ruffle membrane Cell junction After T-cell stimulation, it is temporarily excluded from immunological synapses

Storage and Stability: -20°C/1 year

Gene Name: PTPRJ

Protein Name: Receptor-type tyrosine-protein phosphatase eta

Mouse Gene ID: 19271

Mouse SwissProt Number: Q64455

Research Use Only