Product Description

CD161 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0285P-50, NCP0285P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q12918

Host: Rabbit

Reactivity: Rat, Mouse, Human,

Applications: WB, IF

All Applications: WB: 1:5000~1:10000
IF 1:50~1:200

Background: Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD161 polyclonal antibody detects endogenous levels of CD161 protein.

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD161 polyclonal antibody at 1:5000 dilution
Lane 1: HEK293T cell membrane lysate (30ug)
Lane 2: MCF-7 cell membrane lysate (30ug)
Lane 3: CT-26 whole cell lysate (30ug)
Lane 4: PC12 whole cell lysate (30ug), Immunofluorescence analysis of HEK293T cells using CD161 pAb at dilution of 1:200.

Alternative Name: Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; CD161

Immunogen: Recombinant protein of human CD161.

Conjugate: Unconjugated

Modification: Unmodified