Product Description

CD244 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4345-50, ES4345-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CD244, 2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NAIL, NK cell type I receptor protein 2B4, NKR2B4, h2B4, CD244

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD244. AA range:101-150

Isotype: IgG

Target: CD244

Specificity: CD244 Polyclonal Antibody detects endogenous levels of CD244 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in spleen, PBL, followed by lung, liver, testis and small intestine, Expressed in all natural killer (NK) cells, monocytes and basophils, TCR-gamma/delta+ T-cells, monocytes, basophils, and on a subset of CD8(+) T-cells,

Observed Band (KD): 42kD

Fields: Natural killer cell mediated cytotoxicity

Function: Function: Modulate other receptor-ligand interactions to enhance leukocyte activation
PTM: Phosphorylated
Similarity: Contains 2 Ig-like (immunoglobulin-like) domains
Subunit: Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2
Tissue Specificity: Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils

Subcellular Location: Membrane, Single-pass type I membrane protein Cell membrane Receptor engagement results in a recruitment to lipid drafts essential for the subsequent tyrosine phosphorylation of the ITSMs

Storage and Stability: -20°C/1 year

Gene Name: CD244

Protein Name: Natural killer cell receptor 2B4

Mouse SwissProt Number: Q07763

Research Use Only