CD30 (H559) Polyclonal Antibody-AP0803

CD30 (H559) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0803-50, AP0803-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28908

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB 1:500 - 1:1000
IHC 1:50 - 1:100
IP 1:10 - 1:100

Background: The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine-rich extracellular domains. Members of this family include FAS, OX40, CD27 and CD30. CD30 is a surface marker for neoplastic cells of the Hodgkin's lymphoma and related hematologic malignancies.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD30 (H559) polyclonal antibody detects endogenous levels of CD30 protein.

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD30 (H559) polyclonal antibody at 1:1000 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: CT26 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: The Testis tissue lysate of Rat (40ug), Immunoprecipitation of the Testis tissue lysate of Rat using CD30 (H559) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using CD30 (H559) pAb #AP0803. “↑”(supernatant); “↓”(deposition)

Alternative Name: Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; NFRSF8; CD30; D1S166E

Immunogen: Synthetic peptide, corresponding to amino acids 500-600 of Human CD30.

Conjugate: Unconjugated

Modification: Unmodified