Product Description

CD352 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0302P-50, NCP0302P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q96DU3

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:2000~1:5000
IF 1:50~1:200

Background: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors.Positive signaling in NK cells implicates phosphorylation of VAV1. NK cell activation seems to depend on SH2D1B and not on SH2D1A.In conjunction with SLAMF1 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage (By similarity).Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A.Promotes recruitment of RORC to the IL-17 promoter.In conjunction with SLAMF1 and CD84/SLAMF5 may be a negative regulator of the humoral immune response. In the absence of SH2D1A/SAP can transmit negative signals to CD4+ T-cells and NKT cells. Negatively regulates germinal center formation by inhibiting T-cell:B-cell adhesion; the function probably implicates increased association with PTPN6/SHP-1 via ITSMs in absence of SH2D1A/SAP. However, reported to be involved in maintaining B-cell tolerance in germinal centers and in preventing autoimmunity (By similarity).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD352 polyclonal antibody detects endogenous levels of CD352 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD352 polyclonal antibody at 1:2000 dilution
Lane 1: HCT116 whole cell lysate (20ug)
Lane 2: HuT78 whole cell lysate (24ug)
Lane 3: MCF-7 cell membrane lysate (30ug), Immunofluorescence analysis of MCF-7 cells using CD352 pAb at dilution of 1:200.

Alternative Name: SLAM family member 6; Activating NK receptor; NK-T-B-antigen; NTB-A; CD352

Immunogen: Recombinant protein of human CD352.

Conjugate: Unconjugated

Modification: Unmodified