Product Description

CD358 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0307P-50, NCP0307P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: O75509

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:2000~1:5000
IF 1:50~1:200

Background: Promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B. Can also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. Plays a role in neuronal apoptosis, including apoptosis in response to amyloid peptides derived from APP, and is required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP). N-APP binds TNFRSF21; this triggers caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Negatively regulates oligodendrocyte survival, maturation and myelination. Plays a role in signaling cascades triggered by stimulation of T-cell receptors, in the adaptive immune response and in the regulation of T-cell differentiation and proliferation. Negatively regulates T-cell responses and the release of cytokines such as IL4, IL5, IL10, IL13 and IFNG by Th2 cells. Negatively regulates the production of IgG, IgM and IgM in response to antigens. May inhibit the activation of JNK in response to T-cell stimulation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD358 polyclonal antibody detects endogenous levels of CD358 protein.

Bioworld Molecular Weight: ~ 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD358 polyclonal antibody at 1:2000 dilution
Lane 1: EC9706 cell membrane lysate (30ug)
Lane 2: A549 whole cell lysate (30ug)
Lane 3: HepG2 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using CD358 pAb at dilution of 1:200.

Alternative Name: Tumor necrosis factor receptor superfamily member 21; Death receptor 6; CD358

Immunogen: Recombinant protein of human CD358.

Conjugate: Unconjugated

Modification: Unmodified