CD365 Polyclonal Antibody-NCP0329P

CD365 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0329P-50, NCP0329P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q96D42

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus. TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production. Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1. TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease. NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma. TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production. Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease. In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma. Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD365 polyclonal antibody detects endogenous levels of CD365 protein.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD365 polyclonal antibody at 1:1000 dilution
Lane 1: A2780 whole cell lysate (30ug)
Lane 2: SGC7901 whole cell lysate (30ug)
Lane 3: C6 whole cell lysate (30ug)
Lane 4: L02 cell membrane lysate (24ug), Immunofluorescence analysis of L02 cells using CD365 pAb at dilution of 1:200.

Alternative Name: Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1; CD365; HAVCR1; KIM1; TIM1; TIMD1

Immunogen: Recombinant protein of human CD365.

Conjugate: Unconjugated

Modification: Unmodified