CD48 Polyclonal Antibody-NCP0213P

CD48 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0213P-50, NCP0213P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P09326

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: CD48 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein of the signaling lymphocyte activation molecule (SLAM) family, also known as SLAMF2 and BLAST-1. It is constitutively expressed on most hematopoietic cells (not on neutrophils and a subset of long-term hematopoietic stem cells in mice) and can be upregulated under certain conditions like infection. Interaction with its low affinity ligand CD2 promotes adhesion and TCR signaling. Interaction with the high affinity ligand CD244 (2B4) regulates natural killer (NK) and CD8 T cell activation and cytolytic function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD48 polyclonal antibody detects endogenous levels of CD48 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD48 polyclonal antibody at 1:1000 dilution
Lane 1: LOVO whole cell lysate (30ug)
Lane 2: L02 cell membrane lysate (30ug)
Lane 3: MCF-7 whole cell lysate (30ug)
Lane 4: PMVEC whole cell lysate (20ug), Immunofluorescence analysis of MCF-7 cells using CD48 pAb at dilution of 1:200.

Alternative Name: CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; TCT.1; CD_antigen: CD48; CD48; BCM1; BLAST1

Immunogen: Recombinant protein of human CD48.

Conjugate: Unconjugated

Modification: Unmodified