Product Description

CD6 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4307-50, ES4307-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CD6, T-cell differentiation antigen CD6, T12, TP120, CD6

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD6. AA range:361-410

Isotype: IgG

Target: CD6

Specificity: CD6 Polyclonal Antibody detects endogenous levels of CD6 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected on thymocytes (PubMed:15294938), Detected on peripheral blood T-cells (PubMed:15048703, PubMed:16352806), Detected on natural killer (NK) cells (PubMed:16352806), Soluble CD6 is detected in blood serum (at protein level) (PubMed:17601777), Detected in spleen, thymus, appendix, lymph node and peripheral blood leukocytes (PubMed:9013954), Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain,

Observed Band (KD): 73kD

Fields: Cell adhesion molecules

Function: Function: Involved in cell adhesion. Binds to CD166
PTM: After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues
PTM: Contains intrachain disulfide bond(s)
Similarity: Contains 3 SRCR domains
Tissue Specificity: Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain

Subcellular Location: Cell membrane, Single-pass type I membrane protein Detected at the immunological synapse, ie, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806) Colocalizes with the TCR/CD3 complex at the immunological synapse (PubMed:15294938), [Soluble CD6]: Secreted The origins of the secreted form are not clear, but it might be created by proteolytic shedding of the ectodomain

Storage and Stability: -20°C/1 year

Gene Name: CD6

Protein Name: T-cell differentiation antigen CD6

Mouse SwissProt Number: Q61003

Research Use Only