Product Description

CD63 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4255-50, ES4255-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD63. AA range:121-170

Isotype: IgG

Target: CD63

Specificity: CD63 Polyclonal Antibody detects endogenous levels of CD63 protein.

Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in platelets (at protein level), Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages,

Observed Band (KD): 26,35-65(kD

Fields: Lysosome, Proteoglycans in cancer

Function: Function: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation
Miscellaneous: Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS
Similarity: Belongs to the tetraspanin (TM4SF) family
Subcellular Location: Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules
tissue

Subcellular Location: Cell membrane, Multi-pass membrane protein Lysosome membrane, Multi-pass membrane protein Late endosome membrane, Multi-pass membrane protein Endosome, multivesicular body Melanosome Secreted, extracellular exosome Cell surface Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155) Located in platelet dense granules (PubMed:7682577) Detected in a subset of pre-melanosomes Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903)

Storage and Stability: -20°C/1 year

Gene Name: CD63

Protein Name: CD63 antigen

Mouse SwissProt Number: P41731

Research Use Only