CD68 Polyclonal Antibody-BS80267

CD68 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80267-50, BS80267-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31996

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 80kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of RAW264.7 cells, using CD68 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts of Raji cells, using CD68 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of RAW 264 7 and Jurkat cells using CD68 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of THP-1 cells using CD68 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: La; ma; Sca; gp11; Lamp4; gp110; Scard1

Immunogen: A synthetic peptide of mouse CD68(NP_001277987.1).

Conjugate: Unconjugated

Modification: Unmodified