Product Description

CD74 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4194-50, ES4194-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CD74, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD74. AA range:161-210

Isotype: IgG

Target: CD74

Specificity: CD74 Polyclonal Antibody detects endogenous levels of CD74 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: [Isoform p41]: In B cells, represents 10% of total CD74 expression,, [Isoform p33]: In B cells, represents 70% of total CD74 expression,

Observed Band (KD): 33kD

Fields: Antigen processing and presentation, Tuberculosis, Herpes simplex virus 1 infection

Function: Function: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place
Similarity: Contains 1 thyroglobulin type-1 domain
Subunit: Nonamer composed of three alpha/beta/gamma heterotrimers

Subcellular Location: Cell membrane, Single-pass type II membrane protein Endoplasmic reticulum membrane Golgi apparatus, trans-Golgi network Endosome Lysosome Transits through a number of intracellular compartments in the endocytic pathway It can either undergo proteolysis or reach the cell membrane, [Isoform p41]: Late endosome Lysosome

Storage and Stability: -20°C/1 year

Gene Name: CD74

Protein Name: HLA class II histocompatibility antigen gamma chain

Mouse SwissProt Number: P04441

Research Use Only