CD79A Polyclonal Antibody-NCP0227P

CD79A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0227P-50, NCP0227P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P11912

Host: Rabbit

Reactivity: Rat, Mouse, Human,

Applications: WB, IF

All Applications: WB: 1:2000~1:5000
IF 1:50~1:200

Background: Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD79A polyclonal antibody detects endogenous levels of CD79A protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD79A polyclonal antibody at 1:2000 dilution
Lane 1: C6 whole cell lysate (30ug)
Lane 2: CT-26 whole cell lysate (30ug)
Lane 3: SGC7901 whole cell lysate (30ug)
Lane 4: EC9706 cell membrane lysate (30ug)
Lane 5: MCF-7 cell membrane lysate (30ug), Immunofluorescence analysis of SGC7901 cells using CD79A pAb at dilution of 1:200.

Alternative Name: B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein; CD79a

Immunogen: Recombinant protein of human CD79A.

Conjugate: Unconjugated

Modification: Unmodified