Product Description

CD81 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4124-50, ES4124-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, WB, ELISA

Alternate Names: CD81; TAPA1; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD81. AA range:111-160

Isotype: IgG

Target: CD81,

Specificity: CD81 Polyclonal Antibody detects endogenous levels of CD81 protein.

Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed on B cells (at protein level) (PubMed:20237408). Expressed in hepatocytes (at protein level) (PubMed:12483205). Expressed in monocytes/macrophages (at protein level) (PubMed:12796480). Expressed on both naive and memory CD4-positive T cells (at protein level) (PubMed:22307619).

Observed Band (KD): 26kD

Fields: B cell receptor signaling pathway, Malaria, Hepatitis C

Function: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. PTM: Not glycosylated. Similarity: Belongs to the tetraspanin (TM4SF) family. Subunit: Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Tissue Specificity: Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

Subcellular Location: Cell membrane, Multi-pass membrane protein Basolateral cell membrane, Multi-pass membrane protein Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.

Storage and Stability: -20°C/1 year

Gene Name: CD81

Protein Name: CD81 antigen

Human Gene ID: 975

Human SwissProt Number: P60033

Mouse Gene ID: 12520

Mouse SwissProt Number: P35762

Rat Gene ID: 25621

Rat SwissProt Number: Q62745

Research Use Only