Product Description

Cdc20 (Q105) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2203-50, BS2203-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12834

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins,Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred..

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cdc20 (Q105) polyclonal antibody detects endogenous levels of Cdc20 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cdc20 (Q105) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat liver tissue lysate, Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Cell division cycle protein 20 homolog; p55CDC; CDC20

Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human Cdc20.

Conjugate: Unconjugated

Modification: Unmodified