Product Description

CDC25C Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66962-50, MB66962-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regμlating the entry of all eukaryotic cells into mitosis . cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint . When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis . The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human CDC25C. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of CDC25C protein.

Molecular Weight: ~ 60 kDa

Pathway: Cell Cycle G2 M DNA Damage Signaling Pathway, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P30307

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only