Product Description

CDK2 Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66254-50, MB66254-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regμlated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1. Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK, a complex of CDK7 and cyclin H. CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2.

Category: Primary Antibody

Reactivity: Human, Rat, Hamster

Host: Rabbit

Applications: WB, IHC, IF/ICC, IP

Alternate Names: CDKN2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human Cdk2

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CDK2 protein.

Molecular Weight: ~ 35 kDa

Pathway: Cell Cycle Control G1 S Checkpoin

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P24941

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only