Product Description

CDK5 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66517-50, MB66517-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons to regμlate the cytoarchitecture of these cells. Analogous to cyclins, the regμlatory subunits p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, with high levels of kinase activity detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no substrates have been specifically attributed to p35 or p39. Substrates of CDK5 include p35, PAK1, Src, β-catenin, tau, neurofilament-H, neurofilament-M, synapsin-1, APP, DARPP32, PP1-inhibitor, and Rb. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway. However, p35 stability increases as CDK5 kinase activity decreases, likely as a resμlt of decreased phosphorylation of p35 at Thr138 by CDK5 . Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insμlt, resμlting in prolonged activation of CDK5 by p25. Research studies have shown accumμlation of p25 in neurodegenerative diseases, such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS).

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: CDKN5, Cyclin-dependent kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CDK5 protein.

Molecular Weight: ~ 35 kDa

Pathway: Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q00535

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only