Product Description

Centrin-2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67314-50, BS67314-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P41208

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

Background: Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC . Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export . The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage .

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Centrin-2 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Centrin-2 expression in HT29 (A), MCF7 (B), mouse spleen (C) whole cell lysates., Immunofluorescent analysis of Centrin-2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: CALT; CEN2; Centrin-2; Caltractin isoform 1

Immunogen: Recombinant full length protein of human Centrin-2

Conjugate: Unconjugated

Modification: Unmodified