ChemR23 (R249) Polyclonal Antibody-BS2199

ChemR23 (R249) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2199-50, BS2199-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99788

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Chemokine receptor-like 1 (also designated G protein-coupled receptor DEZ or ChemR23) belongs to the G protein-coupled receptor 1 family. It is an integral membrane protein functioning as a receptor, possibly a chemotactic peptide receptor. It also acts as a co-receptor for various SIV strains and for a primary HIV-1 strain. ChemR23 is highly expressed in developing osseous and cartilaginous tissue, brain, kidney, gastrointestinal tissues and myeloid tissue, as well as in adult parathyroid glands.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ChemR23 (R249) polyclonal antibody detects endogenous levels of ChemR23 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CMKLR1 pAb at 1:500 dilution
Lane 1: RAW264.7 whole cell lysate (40ug)
Lane 2: PMVEC whole cell lysate (40ug)
Lane 3: HUT78 whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (40ug)
Lane 5: LO2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ChemR23 (R249) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ChemR23 (R249) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Chemokine-like receptor 1; G-protein coupled receptor; ChemR23; G-protein coupled receptor DEZ; CMKLR1; CHEMR23; DEZ

Immunogen: Synthetic peptide, corresponding to amino acids 216-265 of Human MChemR23.

Conjugate: Unconjugated

Modification: Unmodified