Product Description

Chk1 (phospho Ser301) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4595-50, ES4595-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301. AA range:271-320

Isotype: IgG

Target: Chk1

Specificity: Phospho-Chk1 (S301) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S301.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon,

Observed Band (KD): 55kD

Fields: Cell cycle, p53 signaling pathway, Cellular senescence, Human T-cell leukemia virus 1 infection, Human immunodeficiency virus 1 infection, Viral carcinogenesis

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Domain: The autoinhibitory region (AIR) inhibits the activity of the kinase domain
Function: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds

Subcellular Location: Nucleus Chromosome Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962) Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285) Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063)

Storage and Stability: -20°C/1 year

Gene Name: CHEK1

Protein Name: Serine/threonine-protein kinase Chk1

Mouse Gene ID: 12649

Mouse SwissProt Number: O35280

Rat Gene ID: 140583

Rat SwissProt Number: Q91ZN7

Research Use Only