Product Description

Cleaved-Caspase-2 p18 (G170) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1016-50, ES1016-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CASP2, ICH1, NEDD2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 2 p18 N-ternal. AA range:151-200

Isotype: IgG

Target: Caspase-2

Specificity: Cleaved-Caspase-2 p18 (G170) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-2 p18 protein resulting from cleavage adjacent to G170.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain, In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle,

Observed Band (KD): 18 or 35kD

Fields: Apoptosis

Function: Alternative Products: Isoforms differ in the N- and C-termini,Catalytic Activity: Strict requirement for an Asp residue at P1, with 316-asp being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-|-
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival
PTM: The mature protease can process its own propeptide, but not that of other caspases
Similarity: Belongs to the peptidase C14A family
Similarity: Contains 1 CARD domain
Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD
Tissue Specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and b

Subcellular Location: nucleus, cytoplasm, mitochondrion, cytosol, membrane,

Storage and Stability: -20°C/1 year

Gene Name: CASP2

Protein Name: Caspase2

Mouse SwissProt Number: P29594

Research Use Only