Product Description

Cleaved-Factor Xa activated HC (I235) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5189-50, ES5189-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative sp

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: F10, Coagulation factor X, Stuart factor, Stuart-Prower factor

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human FA10. AA range:216-265

Isotype: IgG

Target: FA10

Specificity: Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody detects endogenous levels of fragment of activated Factor Xa activated HC protein resulting from cleavage adjacent to I235.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Plasma, synthesized in the liver,

Observed Band (KD): 30kD

Fields: Complement and coagulation cascades

Function: Catalytic Activity: Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin
Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting
Online Information: Factor X entry,PTM: N- and O-glycosylated
PTM: The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway)
PTM: The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains
PTM: The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium
Similarity: Belongs to the peptidase S1 family
Similarity: Contains 1 Gla (gamma-carboxy-glutamate) domain
Similarity: Contains 1 peptidase S1 domain
Similarity: Contains 2 EGF-li

Subcellular Location: Secreted

Storage and Stability: -20°C/1 year

Gene Name: F10

Protein Name: Coagulation factor X

Mouse Gene ID: 14058

Mouse SwissProt Number: O88947

Rat Gene ID: 29243

Rat SwissProt Number: Q63207

Research Use Only