Product Description

Cleaved-PARP-1 (G215) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1052-50, ES1052-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PARP. AA range:196-245

Isotype: IgG

Target: PARP,

Specificity: Cleaved-PARP-1 (G215) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to G215.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Colon carcinoma, Fibroblast, Lung, Ovarian carcinoma, Skin,

Observed Band (KD): 89kD

Fields: Base excision repair, NF-kappa B signaling pathway, Apoptosis, Necroptosis, Diabetic cardiomyopathy

Function: Catalytic Activity: NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. PTM: Poly-ADP-ribosylated by PARP2. Similarity: Contains 1 BRCT

Subcellular Location: Nucleus Nucleus, nucleolus Chromosome Localizes to sites of DNA damage.

Storage and Stability: -20°C/1 year

Gene Name: PARP1

Protein Name: Poly [ADP-ribose] polymerase 1

Human Gene ID: 142

Human SwissProt Number: P09874

Mouse SwissProt Number: P11103

Research Use Only