Product Description

Cleaved PARP Mouse Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: EM1132-50, EM1132-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: PARP-1, Poly(ADP ribose) polymerase 1, sPARP1, ADPRT1, ADP ribosyltransferase NAD(+)

Clonality: Monoclonal

Immunogen: Synthetic Peptide

Isotype: IgG1

Dilution: WB 1:1, 000-3, 000   IHC 1:200-500

Concentration: 1mg/mL

Observed Band (KD): 116, 89kDa

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Human Gene ID: 142

Human SwissProt Number: P09874

Research Use Only