Product Description

COLGALT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76860-50, BS76860-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NBJ5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 72KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mouse ovary, using COLGALT1 antibody at 1:1135 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of HeLa cells using COLGALT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using COLGALT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using COLGALT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: COLGALT1; GLT25D1

Immunogen: Recombinant fusion protein of human COLGALT1(NP_078932.2).

Conjugate: Unconjugated

Modification: Unmodified