Product Description

Collagen 25 alpha 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67221-50, BS67221-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9BXS0

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/IC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Inhibits fibrillization of amyloid-beta peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 182% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Collagen 25 alpha 1 protein.

Bioworld Molecular Weight: ~ 96 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Collagen 25 alpha 1 expression in HEK293T (A), HCT116 (B), HuT78 (C) whole cell lysates., Immunohistochemical analysis of Collagen 25 alpha 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Collagen 25 alpha 1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: Collagen alpha-1(XXV) chain; Alzheimer disease amyloid-associated protein; AMY; CLAC-P

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 25 alpha 1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified