Collagen 3 alpha 1 Monoclonal Antibody-MB66185

Collagen 3 alpha 1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66185-50, MB66185-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P02461

Host: Mouse

Reactivity: Human

Applications: WB, IHC, IF

All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300), IF (1/100 - 1/500)

Background: The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides . One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: fibrillar collagens, non-fibril forming collagens, and fibril-associated collagens. These subgroups differ in their structure and supramolecular assembly .

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of Collagen 3 alpha 1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 135 kDa

Specificity: Recognizes endogenous levels of Collagen 3 alpha 1 protein.

Alternative Name: Collagen alpha-1(III) chain

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Collagen 3 alpha 1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified