Product Description

COMT Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4761-50, ES4761-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: COMT, Catechol O-methyltransferase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human COMT. AA range:61-110

Isotype: IgG

Target: COMT

Specificity: COMT Polyclonal Antibody detects endogenous levels of COMT protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, liver, placenta, lymphocytes and erythrocytes,

Observed Band (KD): 30kD

Fields: Steroid hormone biosynthesis, Tyrosine metabolism, Metabolic pathways, Dopaminergic synapse

Function: Catalytic Activity: S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
Cofactor: Binds 1 magnesium ion per subunit
Function: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol
mass spectrometry: PubMed: 8020475,Online Information: Catechol-O-methyl transferase entry,polymorphism: Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM: 103780]
polymorphism: Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity
PTM: The N-terminus is blocked
Similarity: Belongs to the mammalian catechol-O-methyltransferase family
Tissue Specificity: Brain, liver, placenta,

Subcellular Location: [Isoform Soluble]: Cytoplasm, [Isoform Membrane-bound]: Cell membrane, Single-pass type II membrane protein, Extracellular side

Storage and Stability: -20°C/1 year

Gene Name: COMT

Protein Name: Catechol O-methyltransferase

Mouse SwissProt Number: O88587

Research Use Only