Product Description

COR28 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78134-50, BS78134-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8L983

Host: Rabbit

Reactivity: Arabidopsis thaliana

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Light and temperature are two key environmental signals that profoundly affect plant growth and development, COR28 and COR27 are negative regulators of freezing tolerance but positive regulators of flowering. They are involved in central circadian clock regulation and in flowering promotion, by binding to the chromatin of clock-associated evening genes TOC1, PRR5, ELF4 and cold-responsive genes in order to repress their transcription. And also are direct targets of CCA1, which represses their transcription via chromatin binding.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using COR28 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 30KDa

Specificity: Unmodification

Alternative Name: F17I5.170; F17I5_170

Immunogen: Recombinant fusion protein arabidopsis thaliana COR28(NP_567946.1).

Conjugate: Unconjugated

Modification: Unmodified