Product Description

Cortactin (phospho-Y466) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4779-50, BS4779-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains mμltiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Cortactin (Y466) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr466.

Molecular Weight: ~ 85 kDa

Pathway: Regμlation of Actin Dynamics, Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14247

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only