Product Description

COT Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2031-50, ES2031-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MAP3K8, COT, ESTF, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human COT. AA range:256-305

Isotype: IgG

Target: COT

Specificity: COT Polyclonal Antibody detects endogenous levels of COT protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in several normal tissues and human tumor-derived cell lines,

Observed Band (KD): 60kD

Fields: MAPK signaling pathway, Toll-like receptor signaling pathway, T cell receptor signaling pathway, TNF signaling pathway

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Cofactor: Magnesium
Developmental Stage: Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle
Function: Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein
PTM: Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily
Similarity: Contains 1 protein kinase domain
Subunit: Forms a ternary complex with NFKB1 and TNIP2
Tissue Specificity: Expressed in several normal tissues and

Subcellular Location: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: MAP3K8

Protein Name: Mitogen-activated protein kinase kinase kinase 8

Mouse Gene ID: 26410

Mouse SwissProt Number: Q07174

Rat Gene ID: 116596

Rat SwissProt Number: Q63562

Research Use Only