COUP-TF II Polyclonal Antibody-GCP118

COUP-TF II Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: GCP118-50, GCP118-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: P24468

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:1000~1:2000
IHC: 1:50~1:200

Background: Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family. COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes . COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression. COUP-TFI is involved in neuronal development, tissue patterning, and differentiation . COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COUP-TF II polyclonal antibody detects endogenous levels of COUP-TF II protein.

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of COUP-TF II polyclonal antibody at 1:1000 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Rat Brain using COUP-TF II antibody at dilution of 1:50., Western blot (WB) analysis of COUP-TF II polyclonal antibody at 1:1000 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: COUP-TF II knockdown A549 whole cell lysate (40ug)

Alternative Name: COUP transcription factor 2; Apolipoprotein A-I regulatory protein 1; COUP transcription factor II; COUP-TF2; Nuclear receptor subfamily 2 group F member 2; ARP-1; COUP-TF II; NR2F2; ARP1; TFCOUP2

Immunogen: Synthetic peptide, corresponding to Human COUP-TF II.

Conjugate: Unconjugated

Modification: Unmodified