Product Description

CPE Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2039-50, ES2039-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: carboxypeptidase E(CPE) Homo sapiens This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CPE, Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin convertase, Prohormone-processing carboxypeptidase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CPE. AA range:271-320

Isotype: IgG

Target: CPE

Specificity: CPE Polyclonal Antibody detects endogenous levels of CPE protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Colon, Eye,

Observed Band (KD): 53kD

Fields: Type I diabetes mellitus

Function: Catalytic Activity: Release of C-terminal arginine or lysine residues from polypeptides
Cofactor: Binds 1 zinc ion per subunit
Function: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin
Similarity: Belongs to the peptidase M14 family
Subcellular Location: Secretory granules of pancreatic islets, adrenal gland, pituitary and brain

Subcellular Location: [Isoform 1]: Cytoplasmic vesicle, secretory vesicle Cytoplasmic vesicle, secretory vesicle membrane, Peripheral membrane protein Secreted Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions

Storage and Stability: -20°C/1 year

Gene Name: CPE

Protein Name: Carboxypeptidase E

Mouse Gene ID: 12876

Mouse SwissProt Number: Q00493

Rat Gene ID: 25669

Rat SwissProt Number: P15087

Research Use Only