Product Description

Crk-L (phospho Tyr207) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1517-50, ES1517-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CRKL, Crk-like protein

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222

Isotype: IgG

Target: Crk-L

Specificity: Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Skin, Spleen,

Observed Band (KD): 39kD

Fields: MAPK signaling pathway, ErbB signaling pathway, Rap1 signaling pathway, Chemokine signaling pathway, Focal adhesion, Fc gamma R-mediated phagocytosis, Neurotrophin signaling pathway, Regulation of actin cytoskeleton, Insulin signaling pathway, Growth hormone synthesis, secretion and action, Bacterial invasion of epithelial cells, Shigellosis, Yersinia infection, Human cytomegalovirus infection, Human immunodeficiency virus 1 infection, Pathways in cancer, MicroRNAs in cancer, Renal cell carcinoma, Chronic myeloid leukemia

Function: Function: May mediate the transduction of intracellular signals
Similarity: Contains 1 SH2 domain
Similarity: Contains 2 SH3 domains
Subunit: Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4

Subcellular Location: endosome, cytosol, cell-cell adherens junction, extracellular exosome,

Storage and Stability: -20°C/1 year

Gene Name: CRKL

Protein Name: Crk-like protein

Mouse Gene ID: 12929

Mouse SwissProt Number: P47941

Rat Gene ID: 1.00911e+008

Rat SwissProt Number: Q5U2U2

Research Use Only