Product Description

CRK Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66546-50, MB66546-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CrkII, a cellμlar homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases. The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk. The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins. Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase, IGF-I receptorand EGF receptor. Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecμlar folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: Adapter molecμle crk, Proto-oncogene c-Crk, p38

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human CrkII protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CRK protein.

Molecular Weight: ~ 42 kDa

Pathway: Regμlation of Actin Dynamics，MAPK Erk in Growth and Differentiation Pathway(，Death Receptor Signaling，SAPK JNK Signaling Cascades

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P46108

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only