Product Description

Ctip2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90342-50, BS90342-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9C0K0

Host: Rabbit

Reactivity: Mouse

Applications: WB IHC IF ICC ELISA

All Applications: WB 1:500 - 1:2000
IHC 1:100 - 1:500
IF 1:100 - 1:500
ICC 1:100 - 1:500
ELISA 1:5000 - 1:10000

Background: Bcl-11a (CtIP-1, EVI9, B cell chronic lymphocytic leukemia (CLL)/lymphoma 11A) and Bcl-11b (CtIP-2, RIT1, B cell CLL/lymphoma 11B) genes play crucial roles in lymphopoiesis and influence the progression of hematopoietic malignancies. Disruption of the Bcl-11b locus is linked to T cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T cell lymphoma. Bcl-11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Bcl-11b can interact with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. Bcl-11a is essential for postnatal development and normal lymphopoiesis. The Bcl-11a mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with Bcl-6.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6. 0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ctip2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Bioworld Molecular Weight: ~ 95 kDa

Specificity: Ctip2 polyclonal antibody detects endogenous levels of Ctip2 protein.

Alternative Name: B cell lymphoma/leukemia 11B; B-cell CLL/Lymphoma 11B; B-cell lymphoma/leukemia 11B; BC11B_HUMAN; BCL-11B; Bcl11B; COUP-TF interacting protein 2; COUP-TF-interacting protein 2; Ctip 2; hRit1; MS1029; Radiation induced tumor suppressor gene 1; Radiation induced tumor suppressor gene 1 protein; Radiation-induced tumor suppressor gene 1 protein; Rit 1; Rit1

Immunogen: KLH conjugated synthetic peptide derived from human Ctip2 : 331-4630/894

Conjugate: Unconjugated

Modification: Unmodified