Product Description

CUL5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7559-50, BS7559-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q93034

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB: 1:500~1:2000
IF: 1:50~1:200

Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/Cul-1/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CUL5 polyclonal antibody detects endogenous levels of CUL5 protein.

Bioworld Molecular Weight: ~91 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CUL5 polyclonal antibody, Immunofluorescence analysis of U2OS cells, using CUL5 polyclonal antibody

Alternative Name: CUL 5;
CUL-5;
CUL5;
CUL5_HUMAN;
Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1);
Cullin-5;
Cullin5;
VACM 1;
VACM-1;
VACM1;
Vasopressin activated calcium mobilizing receptor 1;
Vasopressin activated calcium mobilizing receptor;
Vasopressin-activated calcium-mobilizing receptor 1;
Vasopressin-activated calcium-mobilizing receptor-1;

Immunogen: Recombinant full length Human CUL5.

Conjugate: Unconjugated

Modification: Unmodified